spa typing directly from a mecA, spa and pvl multiplex PCR assay—a cost-effective improvement for methicillin-resistant Staphylococcus aureus surveillance
نویسندگان
چکیده
منابع مشابه
Classifying spa types in complexes improves interpretation of typing results for methicillin-resistant Staphylococcus aureus.
A total of 382 isolates of methicillin-resistant Staphylococcus aureus originating from three Austrian regions and one adjacent Italian region (Vienna, Lower Austria, North Tyrol, and South Tyrol) were typed by DNA sequence analysis of the variable repeat region of the protein A gene (spa typing). The strain collection consisted of arbitrarily chosen isolates originating from clinical specimens...
متن کاملEvaluation of spa-typing of methicillin-resistant Staphylococcus aureus using high-resolution melting analysis.
OBJECTIVE spa-typing of methicillin-resistant Staphylococcus aureus (MRSA) has been used widely in clinical diagnostics and epidemiological studies. The aim of this study was to evaluate high-resolution melting (HRM) as a rapid and cost-effective method, to replace DNA-sequencing, for spa-typing in a global collection of 50 MRSA isolates. METHODS The polymorphic X region of the spa gene was a...
متن کاملMolecular typing of methicillin-resistant Staphylococcus aureus strains by PCR-RFLP of SPA gene: a reference laboratory perspective.
PURPOSE To characterize methicillin-resistant Staphylococcus aureus (MRSA) strains by molecular typing based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of spa gene and to assess the utility of spa genotyping over bacteriophage typing in the discrimination of the strains. MATERIALS AND METHODS Studies were undertaken on 125 MRSA strains representing the mo...
متن کاملPhenotypic and Genotypic Evaluation of Methicillin-resistant Staphylococcus aureus Strains Isolated from Children in Tehran Children's Medical Center, 2016
Background and purpose: The aims of this study were to evaluate the antibiotic resistance of Healthcare-associated Methicillin-resistant Staphylococcus aureus (HA-MRSA) and Community associated MRSA (CA-MRSA)-resistant strains of Staphylococcus aureus strains and to investigate the frequency of different types of spa typing, SCCmec I, II, III, IV, V, and type IV, among the strains of MRSA isol...
متن کاملMolecular Analysis of the Presence of pvl, spa, and mecA Genes and Their Correlation with a Range of Antibiotics in Staphylococcus aureus Collected from Burn Patients
Background and Objective: Staphylococcus aureus is one of the most important human pathogens and common causes of nosocomial and acquired infections in the world. The aim of this study was to investigate antibiotic resistance in methicillin-resistant S. aureus (MRSA) strains and the molecular properties of S. aureus strains. Materials and Methods: For this purpose, a descriptive study was perf...
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ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2008
ISSN: 1198-743X
DOI: 10.1111/j.1469-0691.2008.01995.x